	   SnapGene      ÊacgcgtgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccactgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtATCGGTtaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaattcaaaattttatcgatactagtGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTAGAATTAATTTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACTCATCgctagcgaattcgGCCTACCTGAGGATGAAGTATTTCAAGAGAATACTTCATCCTCAGGTAGGCTTTTTTggatccgcggccgcGaaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacgctagacgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctccgccttcgccctcagacgagtcggatctccctttggccgcctccccgcctggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaaaataagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagacttttgcagagacggcccaaattcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagctg  E•   ,   '      '   ,   -         *            0       -   -   (   2      *   -   +                  )               /         )               "                        ,         -   -   -      !   0      )   0   /   *   4   '   &   '      *   0   5   &      %   !             .   .      .   +      %   1   K   )         &   *                                  "            $            !                     $   $                     "             &   ,   *   !   !            $               &   '   *                  &               #                  "         (      #   "             3   #   "   #      "            !   )            )      !                 "            8   $                        '   "   "   F      .      1   )   #      !            "            /   5   "   !   *                     )   .      '   &   '   "         F   #         )   .      -      .         &        , ûQR}~Ú%§!"#0			#	J	€	�	×	LM+,þ|äÚGÉ}¹Ë¹rs' ¬½Sf«î½-K�{¨1	T	‚	œGq…HÊÇ¢r|Iˆ@t – é 8C&$$	4	K	|	¶	µñÊÿRÛ~#‘Ì¨²Ž™' ü¢`°ÛöŽ÷Ø	i
\hwÂyÍßN„üdÛ-ÄÐ}åò"]vÒºQœºER, I 	#.T¬­°³Æõ4L[ˆ=Í 	ƒ	€
&Mc»il� ”æë5rO[Isf‰- i ˆ �¾g€]r.Uÿ0Okuè»2tU	v¿†øLÄÕú€ÈýŽÎ«2JZA ¡ 
G„|ô™ÈÙñ“Ó<ºË­†]£¾}Ê¤�u ¦ ­Öï¾Ž©6y.‹Õâ¤ø^�ßHpbÑ* f q 9Êê5	B	}	1
YsvÜDì1 TkVÜQ÷ ¶0W3$@I©³òM * t jx‘ Eþ%»L	N
¯ÖÓ›°ê@Z•Ü	*j� ó rD'I·	
YŸ"x\f«Q¸3´ó’Íš — ê %	_
¶òËS††ýªãÁl0  öý aºE	u
ð
H]`™25hÎÿ
.‚°îQ¼N^”­TsÐ��[q»R�¯Fk‚  Œ ï£Üí÷wø)	<iØzà…ÙýçÅÑI~óñr%Où6Ì- Ü&Ý±ù@»c¿i	Ù	•
ÃÉç€èÜ`5:æë$‹#|ÐRŽ!Äw¬o»¿1- xÈ™~³�ŽÐ
j
xX\O*eALdz�¦ñ=a€“Æ$*3^s<êõ¹ƒÓÞ)S¹( ôù$p®±»®¸>¥Œ—!	z	Õ	ÿ	f,V‰júìR3J"(MP\LO“#t'2 J L Â r/13>UL´Ç“ÎÐÒÔÚÃÅ©	=	„	c
	'dí#!;ÛBHnJ°œLÚgá 3 A
ö\‰q
-NŽ6Pç6µªDæO©ü’6bã* 
Æ@­v&5Mü�
T�¶C¼-m±½U•õ1ks:¿ê—â2�×' ŠÓ- j ‰ ä+Ú^sÉV¼¸ÁÄÇ�Ö	?	Í	Œ%&w[EÅ�É±ÎífßÏÞÔ3„B+ N �³¹¿h�…Ñ 1•Ñ3PuV	†£5ù5›ÖWþU“¬¾[»áøL÷n ² WÎ/òPv¿ÖàéPl¼7]Ïˆ«#:7ÃNKc° ª §³ì¢lÚð0	†	£	Ã	;
DïHÆÀ‡MÚû�” 0 5c…š\	‰¿s"æC‚v Ä Ý HNl¶ï6}ƒ´ÇèÑÔÌl®dm”~¥ b ¢ · qé�[”§=‡J¤²³Ø…) y ý t] àõÉÚáà
)d”£¶òŸ=»û=fÝ^iq¿Ë4‰›ÉËÜ I “ § Ðouûüý
/þÖWZùüLiI²p/B„ïFi©¶ œ äðUg¿ƒ�¯ÖÜ?NŒžáàù^_ÒU	V� d×Ù7zŒ
k„«žèÍdxcdT/ ø }®‡¨“ªe
¨
ì
€¡¢ã2¥¼_rqáaæ�.ž^/ g r �ž+ý‹ ËrõC	~	/
KZtw¹Ýg‹Óï�éÑ;OTö©RÎ´·1ŒáU bÌjîŠúZ	¡	ê
½Ab’¡ËFK™èà!-4%AJõ  OQ`‹;b	í2W`0¸ºÉô—Ïø_XåSª´ó) Y } Ò CS:=—H+ë6	“	2
/8×EÝUl€Y¬¼Ý&Œ¡Š¨ŸôæåNy¾   Lá+àø:F<ÿ¼˜	øµ
Hçúš
+a  + m kƒã^ÝIM	O
Ô~Ñ± A[€þ×é{	�¯ Ê Hž’!ÿT ‹	°êþ#ÀXœë–ÖÝôkU´  u y^&ïd	(�8i×M8Ü" ]É(X	
R¾#8Byï2Æ¦ÿM=@¥ÍÛd×`¬=‹¬ 5 ô s¿Ís¿Ú
]g¡¹ô�+”µçô“ ü	JU�,¸	S
Ä
É
bS¬�Rv4<â2f;› <E§	
Ð
PW}4Z ðŠ˜ó%«B#€²âÎ &	_	›	
`
ÃœØó#­ÚO
·#‡þ\c¶ ‡Dñ·CEŽð‡K«ZÐDtm˜¢¤ 	  ˜ ë Y�ÖI
¾
³Ì)ŸTÂÌJ€9 ™ò2w 	B
q
Æ]LY°µQäÂ, þ!O—ÎH	^aå3³ö)/Wï3½En~‘EO’™®ž.²Y¢¦°ÊðP ·÷<a†ÔßiÏüøU•U‘2š�\¼SGlƒ  B
@
I6`�e{Ö!›±R�ýVtÌr9 - ¡çéb»þF	í	v
Õ
ñ
šú/¼G’Ô FhƒÕ÷ùcCC_€ŽÑ5ãPÙ6‡Ó7ž 5- ¿ ›Ó¨¼(ORd™œŸäx-ù•	t«áEþjÒòZkÒsvŒDGRÛâú‘]ŒÕ-  # 9 � ð¤.x“½ÌîÄÍøH*	±	®wÂèmÆ wJl0säï@�¼é8ª)7A7~ [ º ?ÃÝ¬Þ	4|>†Hny�žÈ/4P‡Í! T ëŽûY= 	=j‘Ùû={¾Äí$r–Úmˆe[ô&ýöÕçÿ0 áÞr«úE[¼Ê	ç
V³:Ùo�¿aÙ&fÙÜì%…õ�,}�ä2ê'ŒÔ¼2h{Å '¯ô²T‚ù
ÄèKQÌñT$}×<ì£ÅI\) '8EÝa7¨ABdÀÐ	Ÿ
®
ÊE�ä;Ü÷¥BI�¡®]Si¦Óxp%sÀ0 Ù Èç+åj	Ú	Z
x
–
²
¾éÝß6Á’ç(ŒuÑ¨aq‚¢"E„�Þå‡¥­_z¹g¡`/ _ · Ñž	¼	ó	

!
F
’
×
1yY]bÐßfux�8Me…×òûIÉˆÇ%+Á£ËÔß>…* yš¿	
 !¸ÿ³1²N�”©§„·5(=ëölyðÇ'C‰è")d•ºÀ4 @  Ô -<ÉgJ��8	o	$
+
4
k
ƒ
¤
ó
ö[š¬I{‚äÌÐ,�”¥ÊÒm–¥.4_Öß¹7ºPõT' U£´í	ï	ö	œ©éP+>BŽ—¸W{>Õb«¾ßëõ t'*„m*”& «¼ú|ª¼¯z¦/�"	{	Ö	gvûcÃÉã!„È¡uÑHP�Ê›Dq˜' H ‡ Õ-	F\q¯²ô3T<ºx˜Ø-Šk“]4KNÔùa…Ð\½É1 p HÉºA	M
^
rëSPéü”* ‹ õ%wÜ}¹ì?v²º¾ 
˜WyíHð#)Q]M¬éô rœ$NŽ5(0�0 K Á qÅó02?Kou%’pÏÑÓÙÂÄ¨û<	
eŽ, ÚACI!mKNŽ¨Òâ5 M *V„²ÐÙÈ¡ñ”¾ÐÕßOÀÃÆ6\Îª	>	…	Â	î$G"Å4DÂÙÍTeÝÓM“º÷& Ã ü s4\MkµpîßÆçß
(ˆ¢µ<r¦ú<eÜkIh±Êc²!Û× ’ ÷ |ãTf†§‚’ÕÛ>Md
‹
ü
¡±à`åco^�hŽ% Ñ BNŠ�Ë*–Šií*‰ôY	’	.è«·óEJÎç³‹ ‰àäRžåä½! l KG�*‚ßâ÷]9;îSŸ—	'�7´	"GÕóýÖ7 4 ¾WûTr	R
OQ7¥ïòªóáL"*1“ÚcáŠ   †Õ	š	
Â¶ïJPÿC£  ¶æ AN–…ýä�.² (VÔö2m˜Ë�-1Ø±¡~¥. 8 S š§-w’ú'NQX›žÂãÌGs�­½=v•lrx�Çãî3u‹CF»ü�(†‹Ô. Ø 7Ç®ó`6æ§É	Ï	Y
æ
UDJãn€¾‘ôt \Ö;Œë+¡ƒÝy¸_rgÄ ,f‚
‘
¨·õ=–zl•ÀÕ)xïÆÊ“. G o Š »,vÛE[¹È¹w×@	—qêx’È\ï «èóÏ[ ¼Ð�È4GOCp€+ òƒSjt…
$ð½ÏÔÞNÂÅ5[æ§‡�¡+ Ä¥ß;3Øj]dg ö k Ð J�ÊÞúRž	X	‘	Q
&³FÍI²àòßÕÙB�ä‰% R +6µåM¦_•Wâ¦Î	X
å
r<'y”ŸqŽÆÔ‚Ê
îB°…}’Ã1 Q 5´º‚DR€Ý	#Ó4ZåQvW	W
–¤ž2p�ÌÓª×çòiZ»ñ¯œFãoK O P ‘ijk†‡ˆ”Qzåæ !"2ÆÒ'deqxó$1š6úæñ;<ÿ VWXY”¹ºÙÚ¬­®›­¿+,9CDE¼½âM±) ’l‰�ç×çc–ò(jryô%2‡›7û6l€‰xœ=ÔÛç:\¾Æø å À•Ò{3ÇUfßO	´X• Á®À-ÂùN² lQ×ó33áå7c½ã4Ü‘;]±&  â 2Šè%Ø0`¬Qm½8Dõ¬8›;muŠ³¾.Ow�Ld{¿Çù* ³ Xm�ÂÏè†¦—ž¸áê)^kszÊÐÔ&‰,	Êœü'�y�®ùÕ “ÂódwÀ$ˆhü€8Ä>èì - æ ´ñ1	¤	³	E°ÔIL2@Ï�:†¯’³ % ; � ¨–Ó|£¿m›ÛJgàÄ	„yo¸Û‚.Ã  mÎ4VKP	<
¶
Ç¸Á€Ö+NµåïC¾–gë•ÁüÄ9 « Áù0íÈŸ‡	ð"ŒnˆüYU Â>ëúO ( Ÿ 6d ^†ô4$ÀËJt "d„mñ÷ 1 ¹mØ4››~Š0p1æ8ä5®Ýú’™žD *]	U¼Ï¾ÊÞ˜£ç^²w  > ] R@"âï#W<[ƒÈî ›Im1”7Rgn¾9EvŠÛJ/PŽÈ" ã 3�#OÕÛað%1BÈ	�	Ò9œ)an‹ÊÍge¿MÀ Å ‹™!·ÓÙ#~¦®é>ik�	<öø|ªì  Þ é&­Q„°µö­Õ»´m¯n•x‚ek¦ú c £ ´ ¼ Ç 8ÃéŠµr¹å-	¨ô�´WžúZF¨�–Ù$ „ JY‘Ðñ‡˜Ÿâë*‡ê_lËÑë'•ö1ˆK³Ö~þ_† Ì §!9{”¨ÕV\¢�	%‚’Ø¥=´‰¬¶Ù n¸-€åtžŠË²ý>( ù?z¯uG%, z ^ÁŠ€â	* eˆ©®ä>Æ9?GiRÊJ! ÖAßx;6¤‰ì>{ºÇ�žÅ6¶é¸Šê¥�"Ï þ ”¡ÅeömÛà	•ó¼ü>gýÂíß_rÀí,5Ì
 uÃýôá´Ê
á
§4@·% iÞÚ¤¼<jÌFñûœÝ ” ç ívòü2	´	FÇ	0ÿJ×Býºq¶Â{pzXGj· . w ¨ µ}²ë*TXú/³3½0b‘k‡%§´ øý¥	¼
»?Õ[MMJW›;°ðª   Ñpë	
�
÷
þ
ý±˜k�ÒÊ×jAÐCâN…£]b“$ � ©ñ(�{ÀÓn�°×Ý(K	Ç	Ý
�tpáÀ_ƒ¦ÓL/p$ E à å÷h}À„¤œ¤ÜSbho·ãñ…Ÿ:˜úíj~‡¬¶9Ä� & < � —Ô@O	 ¬â¹ôÜ—Œ¨×
Y V{¢¯áÅ	z—´`ÈVW ¯ $ë�BÚQ	f	=
¬]uöO�›uo|ÿ0ýÞ: n[W†8…¹�×dD¿ÊhÅ `	L¯Ól&Ÿé,Oeeì–  eØÏ5±{�
·
ÈÂ,Î¶æðy¦—Uí:Âðgm" V ‹Fˆ	f
í
9N}›¶|Üî3™¦ºŠª@F`ýª/$—©_„Ñü  ¬ ù ÂúXˆî?É„�“Ð’½À‡âbo!|Ã?ûé ¯©€˜“û	‡
¢¯ÛÀï#g�Š8rÙ±÷Æ(ëP  ~1ý” «©
?lßñÆ8£äQoˆso‰çZžVŸìø& 7e¡í,BJ_Ù$Œõ ö	S		[ÁxÌÞƒðÏ{ñQpÙ\"?�, h   ¥ Ž¬Åþ/NZjtƒ‡çÌ5sÇ
%LºhŒÔuê’ÒÞ;÷Z�ª* ) e s ò i�D0
XuÛžj®!UwÕ[Òeª…~µÛ)2©âViò2! ŸÇøbD	h		Kc�¥<P#Å{ÏSÏq¸‚nÝø®¾j! 2 @	º­·‹–þ	
µBT2j©G9LåéáN¯û"5KÙ&Ÿà ± ²ãÍë»ÙkïOœ/	¢	Ì	:
C‹…¢9š6°ìÞbƒ¯Kö Ÿ5œ[	¾c“p“šÿB › cntØû§
ë
 1qªhøçÌ
w.ƒ
S.îEU$ PR_aŒ:<üGÿq.
é
î Ð:X_/¹»ÈÊõ¨–˜ß§,ôx   É à]Üc	é÷L¿Ðæù™`Tè³zÛ® \Ì;È¾+Ã
Ï
V3½aî1ÅR� —;
?¤æÖ_«< Œ^	p
(D�	¯Ë"´8YÏ˜vµ ;Þ?
Ô
ùFB�üU‘�4â5XÉï4O&  " Z ¾ >˜Ë<,«”	ú<Ã#DqÁmZ/Y�j.?Žè©õ\æ}' &Dàª*DSZA­
±
²9ØÞÀÛðö%eÛ'S„AH�­¢é‹H$f z* ? ^ ~ ¶ Ó ;TIì7	»	 
3
£
0ÏV�ãƒ¶ÏÔ4‡¤½Þ
'¢OBl=ç„¿ † ó2;Gu½L
ƒèû›É ö p)[±ÏâJenío¿=ÁÍ ;	Š
û
ù_ÙbÌÉb¹   M…œá•V8Shì:ˆí™	¶î¤IK!0Q 7 × ™,ùF„ wÕ‹ÜŠ,·� n Ï *Lu„M�	P
Þ:“\ŸØNõ|
ˆ& ä 4Ž“PÖÜä
bñ&YÞ#0N	V
�oŒËÒhÓØæðÿÀšÁ° Á$_«·2CÉÓ
à&bâø3²-€ , lšJÓ·Õ*äîÎB�½fêÃ‘  Ÿ¸])?/»Îÿ0—ùlöVÇí Œš "ÒÔÚ“"$f¯hjŒ	Ž	(`fõ÷# ƒ Æ Ë I7‰´ä,“§êU¡±ó³$V>Y×<}€•«µ ^ ‰ :lë=ÁÆÙ£�»ì;FhÞµ+·ðéŒ! v ±÷»
ö
Æê)Sj.ÉÖV¼µÁMzjoya D ß ö'z¡®'Ran¶ðÜ

9—ìi¥ÇK–§  ê_…®e	N9flÝ E’ú	†
®ÏÞM‘‡Øn©°ö{–ƒÐû" d ¤ ÄAI#MYsæCaÆ	~
zðÄY[Ú¨hÜ©­>Œ ?ê¶º×N.	
S©¨M¯oØa‚®  È ^9:‹þpo
Â
Ÿè9CKÇQ§®Ÿ:	>¦Î—uÚ­( ! µ ½ :ß©Ê);Yªº	¢
°
¿õ$d‚µÓ3G£.Xiû T>N4AGek 6 … õ „ZÎdòìtëÀÌìÿ^í‰¶¸# Î )Kt’À™ª¶LÒ"/U
¶ßÝ
’÷2žÒ×å,™Â‡"  ‚ Ñˆã+ˆ~(g/'U_Å¢º:g´¶ïèõ‹”ÿ C  `mDÛ
–Èëh¨õLz•Ï`  9¨":ýX©ÖÅ¹	n
¡
Á
žçcÃF­XhSÙ9=n§3g­ Í ƒc
K}!.fT
ÇÞ.Ü	ì&TÑäµî˜Š·3 ‚VW| -ÂÃÄr	s	«	¬	Ê
Æ
DEë“”•–UVWg5678u¦Ðã'uvœ# bŽ•W]£t	­	Å
Ë
—¡§ƒ—‘w¼Õv>(wÚ.<�" o¹æX‹®	Ò	Æ
QÕæX¯~5¢^¨{‹Äôú?$¾¶ã/=&Y# G¤.��u£¤±è	¥Gn˜ò(�=)°„˜&ÃCrvx- Fc^¨	
Ì
Ñ
ÌÌ
+³?P¡ú½Ö¬w)H³" =–Ÿu	öƒ^Ì[¡
ù˜¨þuy™ñ@’°ekx™�qÏÛž ÚpŒ'	ÖfÙå?®8é_¸1:„ŒÅõû¿·>·K Û Îçù�YÓ	a
+R!�¯ß$P@ä { ‹`	Ç
‰ÄÛ|$ˆŠÿH@]SadËò0! _ºÂ€ã	œ	¯	Ü	
çYª°öLsô6¶£Ç*©o{j%5'Z) zˆE×¥�¸ò\
�
¦HóÀF�¯ãñ ?ŸÆLð'‰/¹wÑŽ™£Ð. 0HBjàv¥´
o)žŠf™±»¨Gb·ê‹<uÆy 7/¤<}²¥íÄ¸È‚ˆÙ¬DsïE£¦iœ Ñy‚é	7™¸Ð>D*|…™ªòs¤7à™Ä§[ë#n¥) ™ ð ÿ ZgŽÆ×-hÜ
Í
Ò
ÍÍ�@QŽ?YhyÃÐÍ7àç�6€*I Sx —5dJ
¹
Í, ¢Óxî:ó! 
   ¢G_n©	¿
–Ê´,—ýÃcK­sÁi´ÍoW  ì î •ÊÌf÷á	ã	˜
ô´* ½UWwþû’¾×î`-	Äš3 Ëçl	C
r
¨_¡™ÿš¢èòý¥f$š¶=ò|ÆÐ éâ	
š
â
5Çëj±A±½RG¯øüÞ  Ä>õ³ µå	7
÷AÍ~¢Ã”Mv“l”kÍå»Ãr" vþ›—	v	'
z
„¸&^\á&ú©¯Zz¿Í.ßÛy†ÀW‚�ÜbŸ • è P3	I	µ	h
K´Ú*ý›FFÆ|q{¸‡§±¸Q "óbáð£÷å¾o¹Ã¬üÍY£e€ qwýé
C0X~P…š¯·ö?YŸ/~³À?HkñL8 Ûîÿ˜�Ï(	t
”
ï
;G_È×æ
14[g Øç)æþ@_Œ¯ð49»ê`Š’2r;�“Ã«ZpË¸$ ø=¤	y	Ô	b
,Sì"+Uˆ~ù5°¼:Oô0Q´'¾–1’›Ö‘a& © ¦¸·‡Õ%Z4V’½àm / a x Ü b¶è‚³ZàÐž3lˆÈåH„ Ï~®új�ýVYƒàû»KA]œ-c]T¨µ  X | ùa	 	J¼7@a‘¸ÖfŠÒœÜNS%‹Í^Tó þHŠ	hý}Wÿ Œ¦	
È
Ù
|Aþ‰u $œA<eÌ±:« XCðA
H
½
Å\›×"¬²ÙBNKXžÁI[bsñ1—¡'  è`ÍÓG	ì	
_ûd»÷Tz‘ÓÕõEgšøbD}œBD
O™†Ò68Ÿ" ¹ Òê»�c¼Ãÿ°	Ý	3{ªìlGi‡dvkÑQÚ7á@Ôþ6" q�äw
ž
ø
P½ËØ¤Ø§`hp|�D�¥Òã1ä&†¤^Ó[F ¢	n	�	¾	î	ò	õ	

#
*
E
Ö
ò
ÿ
 ›þ²è0Za™«±ÞHMtw€Œ“¨7„·ÖúHÈË+¤ªÉÑÞêôÿ-&¸6kô&ˆ!(c” ªÔ{©S|ëy±.¹]
ubÂGâÇÓø`q„M0�š/—. ‘ À û {ÄØ¦� o‘‘ØÞLúÁ	Þ
F´ÿq°dä@ ClÁM�§HS±ÜÒ’�ÑÖ ‰œF½))Ô)ó	–	Áò!îñÔòŠˆþ)ãà¢¼1 ò‘Peš�ÁåËÈ	�
§ãIŽ¬âô<u”kÓ�ló[* í(twE0ºÅÉxû^q¤f) F Iñ¥Zi”áï¥ÎÝù¸¦ä
q†p *Ÿ;Ãé‘Çxî²H	AcŒAÇ‘Â# æCy~ÅTcipwäò•™1kˆ³w©åð:¸«*8BÅ  á 1Ák…¥�+	Éûgäšt¼­ë�½vz! $ : Ž ø/¾ÍžI²	µ
ƒ¯xn!‹Km1·?Tê9…=û8 ' = \ ž š#}Ê!TIoî!ÉƒÝ­˜¢ð�Z ˜0AP¥­=‰ºÉÚI�j©ë » ð8†ä€‘Ÿøõ0EtF§ˆØý$+" Õü@ÄÞ~³	ß	
5¦3?‡¨­ãÅz¹5E~úQ¤	Î ì|ê	
œ
ºü>—A�Ñ¹áaš¢\W$¦ �Òz£â	
	Æ	s–«}†«³µó¿8‹ÖXo  ® #œZA°Ò%\tõ+Œšt¥n{Éìþï/ U WŠü >ƒ—8>k’Ú|šµ¿Åî7{ÛíPn˜¹‰©¿?Ef†‰7#Å'¨÷êè5 ñ hÆì«�Ø÷.i‚R	g	WÚ‚b€¤Îi‘­ vÝ:ÔPZozÑÎØ~p}(�1!½i" š °  ±âžs¬•Ë	ý	9
¦
BŸ´„g÷i	8v-ë‚	RJþíTß! [ËßÇûF\½Û-
Î
è
'^Œí.öÄŽ•!–³å7+Õy;* %CÝ
>
¬
Ó
±ù;ÂøCpÎÀÚÎÚ&ARƒ†�@Žœ	-~�¡3áè�ç 3| Ö o˜(°U‡ƒ:	K
‰
ú
‚Ø£ÔaËÈ 	X%~ãáí¼ïô 6\³e’9.ºÍò²Ø=eE*;Æ] õ‘ù	…
º
õ
­ÅÎÝéLR	†-U¤ÀÆyiJ    9>Þ@HLBM`o}
¯
ÒJ¢§-PL®jt  B � (©µCµêþ‘^Ä§9fyÎÁ) �b8a
eÁq	ª	m
 
À
ÅÝæ-– ¦ÂÛCêTf»ÔmtÏí&—t-. F£¢°Ñ	ç	¤ËÔõFm‚—åñ']œ®	Ë	*<øO]tx˜§¯djÃùÂq½µpX Ú ÍÙøÛ	õKrÞµ7è)0nzƒ‰`ñ4' /yiÐ6|·[
Ž
³
¿ÏÃe®˜â·©ñr£§FØßïaˆ˜.¦î;¢Åh›&  í ï f–ÉË4,gâ	—
¸
É�+�–VXvxÏÿÒ6b‘æ
hÃnRV' è²šæk	ä	&
6
y
™
ê}à%Â“®¡¾Ì-çü#…“¿V®÷{ºÅa" è
g
s
“
î
:Z~àæï(¢^‹äü}š‰Â«>XªÌ}od† ` ¥£­¶	x	‡œ}4‚»9NºóJY&\›,ÕÇ`ß W WGï‰	Ÿ	G
Ø
·Ñgš«ØAü@ Àþ#Z–F ¸ ŒÌÒm	½	ñ	ô	
"
)
D
2zO^©ë`cºÝSvy‹§Df†™GauÊ×|›£ÈÐÝéóþ,¦g	%¤·0%˜óà…Ò?Ñý# ú Ãz€êÊÀ	â;“³aÁkcíB)ì÷pñ‡°ÄÈý(ße–¡» Y�ã
p‚”È�ã0²v>S„@úÁ ûï…ã™
" É2S§ ÷H}ùD¡üê#*) ­ "‰›ÿ@–		

›
¹Ñ´6ì@OŽ•ª¨¾s…ˆ¸6‹²¾mz"¡Šîé. °ÅÊªö�”ü	,
8
¥
«
Až°øÁ÷B£hƒ‹Í-9‚Ón”× ,}• ²Qæì Õ ‘�9	ø	„
ˆ
ô
¬Ü-±â`à»Ç)- A € =€ ?K´¡p	æ	l
|
£ôð\›­)Äå•ÑN\sw¦ÁÚ¦éºÂ8ølÎ¼s, .x3h™¶%
5
JqÎ|$Ž­d•­Ë—á/5mˆöÄQU. V¤¢¬µî	w	ð	(
†ê¹å'RŠC]˜¹ãXt|û?Ö{Ï¿Ü["+WYnÞc• !þ•œ˜	oáÈ:R¦?`�r/Šìöu† ƒ íù  wÿ2÷	{
ª
@�«ó,p(¬°[c¬Ààèá+|‡ŸÁ+& ¡	mnªàò…Ç9Q¤¥_‰â'pqª«Îà()*z{ !X…žŸÝ  2l  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        %                                                                                                                                                                                                                                                                                                                 8                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             *                           $                  	                           
                                            *                                            
                                                                                                                       
                           	   	                     D   $            	      	   
   	                              	                                    	   &                  '   F   ,      .   -      )   -   "      Ÿ   Z      &   *      (   #   l                     %                                                                                                                                               8                                                                                                                                                                                                                                                                                                                                     *         $      	         
                *                
                                           
         	 	       D $    	  	 
 	          	            	 &      ' F ,  . -  ) - "  Ÿ Z  & *  ( # l   ƒ  „m  „…  „×  Š  �  —íB�¹sf` ¤ªö�¶îï9	w	x	ø	(
l
no¹O`cC‹Áâ@sÖw‚þ>ìþ¤»cTAÁwWh�ï¬Ót Õ]Òœé.…+Ë)ë(q=&”IÃeçª�¶9	ø	<çŽY²ÃÄ†þ•	í	
+
–
4a‘`äíü>xÔ/a…ŽòenRÌÐ,ˆ”¥%+kÖV™GËç )G

6;ŸÚàTžÚüTÚTÚÚÚÚÚAQðWÓ÷ŸÆ�Ú*˜i &”˜ÈŸßÎ£aX]õŸ£]¿”¶ü	«³«Z
asŒ¶£g¬¨�MN(ªèý f¸Æ¨f ”¿ `™b¬¨'_y]ZLIïá<¡54¯ÀÖ7 ê5	1
ð
<i™ØzÙÿ‚ TÖÜ%¦±U1— ¤	:¯˜Ï[Kc¥ðÅ;Ý¸lryˆ›€xâË	ë£¤Fñº> ð
ý
™Zÿ‚°LIqï(‘  Ü†
Æ�¼ÝYsŸôåYôå¢	w	†NÑö1‚SëhyNUYò¼ôå¥È
ÆØ	NÛ•ås€$‰ñ ð
™ÿ‚[ Ó­¤ëQ	Oì† ße	Cöå¤J u
ð
™.ÿ‚Ô^Ðü ú<e ¬¨©Èê5	i	1
€
ð
<i™ØzMNOÙâèÿY‚”Ÿ TÖ†Üú!%ƒ¬Í«è®>§LFaÅx àÈHê Sc
œ÷º>zY?K£Ìx	|
"‡·ÉÑ@`a�Áâü¸  |n&/×|–Pžçî¡ª=|�» €E
’
í
9Y}Šˆ©ç÷®±j3MÏ[ðá:�—+“	ù;Âpx Ïà[ð…-È]Hh²ž1ng"É _ö
SÆëØ	h�y¼NÛ•ÝUYåòs¼Ÿôå¬œÜY?K|
"É� É–bnVsŒ[ 8Ýç¤“‚Ú	ï	’
®
û6Yéö–Ba—÷wÌÕ(�ˆ¡(•ë@Ç¼PdhÌ"·ÉÑ@¸  |< [  ÜYü sßôÈÙß
µñºú<eÜ]h¾ÊÊÛa~	¨Å›(â#AŒšþÌ|!.T
	Ñä˜`¼l	C
_¢èòý$¶ò
Æsâ«Ë	ë£è¤Fñ®Pš�tEC±Úƒèˆ=ë6_¦Î	Ÿáî<ÝM	b	¡	ÓYþôåÌ_-h÷¥v	7
a
!)žö¡�AÅK­b¬¯œ#¥Á+	  šRX·’
YˆÌÈ	ã×l|ó*0%Ò¤f8Mò%ÔÙ7è0CÈ	ó*f(>‘§FLaÅ{)�2^ü÷„¿7"]îc	7L7Û ò [{eò
ôÈÙñº]¾ÊtàÉà
¶ŸÝiË›ÜzÅªêö�a£Áh9Ón×÷p}–ë ³)ŸT™2ÔÚc
nœgOd™œä:«áo¿jÒÙÙ%…òZ,}�2svDŒú]2{­©³Ži
wNOâ*d¤A�^=apsƒÒãÁ)Rg
å']ãü|šWnc†3$@I‚7™¸>DÄ§­©â¤^pãÁOd™œä«ájÒòZsvDú]:o¿ÙÙ%…,}�2Œ2{û
 2§}ù5c…¿síFÇ	0ÿ×ýºqÂ·ž	F
×
Ðf×· þ—Od™œäÎÑ¼	ó	
!
1^yå3:]«áboux¿ej…Òï3ÙÉÙ%~…‘ÇòZ+,}�’£2svDŒßúY>]Ê2{zÅªêö�g
å'a]£Áãüh|š9Ón×÷p}Wnc†–?ÃÝÞ	4>†y4PÍ"‚ëQ	f	¬uOo|0íñ‡ý(ß¡»„™�uü³
¦3?Eúy·[
Ž
¿®âïˆ. ¬•ý	´i8Jß¬î½�¨œG	]YV (vÓü(ÚF	ˆ	¶B™ª@]¶ýÓGj: y¬•·ý	[
Ž
´¿®þiâ8ïˆ.Jôß‰œ))óòŠˆã¼® #œA\tõŒštn{þÐoý
iB„ÈB@
Ö!±�r ð	(
ê¹RŠC˜tÖ{ÏÜOH	³)EE¦°Pd×·òv¿�z
Œ
#=µÍ7xÃOTÏúýVY›×û"¬ÙKNA[b¨µ™ ÿ z×¥�¯¸hã?7Lšû�ÆÑISÐ ¡æ	£ôð\›­)N\sw¦Âø¼‰ +Ú¸ÇÖ?	w[ÎÏ3B+ bkúIÚ
ê
·CËÔgA€ôZ”-ém�¤s¼ÁÄŒf¢–Ê—cÁ´oW
µOd×zŒ
ÍxTbúê
·CËZ-m¤·òv¿�#=7Ã· þ—ÎÑ¼	ó	
!
1^yå3]buxe…ï3É~‘Ç+’£ßY>Ê® #‰œœ)A)ó\tõŒòštn{Šˆãþ¼Ø›Š85Ý.£±CròXÿ è`ÍÓG	ì	
_ûd»÷Tz‘ÓÕõEgšøbD}œBD
O™†Ò68Ÿ¸ ŒÌÒm	½	ñ	ô	
"
)
D
2zO^©ë`cºÝSvy‹§Df†™GauÊ×|›£ÈÐÝéóþ,¦g	%¤·0%˜óà…Ò?Ñýþ!O—ÎH	^aå3³ö)/Wï3½En~‘EO’™®ž.²Y¢¦°ÊðPu y^&ïd	(�8i×M8ÜØ 7Ç®ó`6æ§É	Ï	Y
æ
UDJãn€¾‘ôt \Ö;Œë+¡ƒÝy¸_rgÄ¡çéb»þF	í	v
Õ
ñ
šú/¼G’Ô FhƒÕ÷ùcCC_€ŽÑ5ãPÙ6‡Ó7ž 5Ö o˜(°U‡ƒ:	K
‰
ú
‚Ø£ÔaËÈy ý t] àõÉÚáà
)d”£¶òŸ=»û=fÝ^iq¿Ë4‰›ÉËÜ IxÈ™~³�ŽÐ
j
xX\O*eALdz�¦ñ=a€“Æ$*3^s<êõ¹ƒÓÞ)S¹è
g
s
“
î
:Z~àæï(¢^‹äü}š‰Â«>XªÌ}od†' = \ ž š#}Ê!TIoî!ÉƒÝ­˜¢ð�ZØ ,7ÇÜ&x®ÈÝó`™6f~±æù§@³�»cŽ¿Ði	É	Ï	Ù	
Y
j
‚
‘
•
æ
Ux¨·ÃÉçDJX\€ãõOn€è*=e¾ÜAL`dz�–¦ñ5:z=‘æë$a€‹“Æô#lt|• \ÐÖ$*3;R^sŒÀÕë+<Ž¡êõ!ƒÝ)x¹Äïwƒ¬ÆÊÓoy¸Þ)_r“»¿1Sg¹Ä ' = \ ž Œ˜šŸ "¸ÒÔÚ]“"$0fAPš¥­¯)=?hjŒ	Ž	#}Ê!T/Io»Îîÿ0!(`Éƒ‰ºÉÝfÚ—­õ÷ùIl˜¢ðöV�j�©ZÇëíøý¥	¼
»?Õ[MMJW›;°ðª " Z ¾ >˜Ë<,«”	ú<Ã#DqÁmZ/Y�j.?Žè©õ\æ}³ Xm�ÂÏè†¦—ž¸áê)^kszÊÐÔ&‰,	Êœü'�y�®ùÕ.x3h™¶%
5
JqÎ|$Ž­d•­Ë—á/5mˆöÄQUôù$p®±»®¸>¥Œ—!	z	Õ	ÿ	f,V‰júìR3J"(MP\LO“#t'Î )Kt’À™ª¶LÒ"/U
¶ßÝ
’÷2žÒ×å,™Â‡p Ø 7¯Ç®ó`©6æ€§H˜Éøý“ºA	¥	É	Ï	û	M
Y
^
‡
¼
æ
U¢¯»Û?DJrÀÕãïn€[¾ë#MSg�MŠ‘8JWrÙôt \Ö;Œë+¡Pƒ±Ýéü”÷›Æ(;y¸°ëðP_rgªÄÛ Îçù�YÓ	a
+R!�¯ß$P@ä  8*     -  É        És"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕkUªlg’>jý†¾¶j6gÔ8¤De¤ZZ‡‰]Õ¤�Vr‘RYjè	*4ªU¦*•`y¶c§Éz&¬jè:™›¢Aí³“°×X‡Æb­D]›vŠ:ªó1A¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌ»Ï¬þÑÄ°nþ óïóàŸ©GÖ�CN$n“ÞªU@àP9k{õÅYFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨Ñ?V²[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øråöšm‡i~eVC&ÜCú²ìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À8:^c´M'„Hßf0ˆV|ÝÉAgÍ´3ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ=N            ‹  ‚  Ž  È  Õ  ×  È  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,Ã<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="shRNA1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2192-2242" color="#a6acb3" type="standard"/></Feature><Feature recentID="23" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2817-2826" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4703" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4320" maxFusedRunOn="4320" detectionMode="exactProteinMatch"><Segment range="4704-4715" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8112-8128" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5536-5552" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5512-5528" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5605-5626" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5560-5566" color="#ffffff" type="standard"/><Segment range="5567-5584" color="#ffffff" type="standard"/><Segment name="-35" range="5585-5590" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3629" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4232" maxFusedRunOn="4232" detectionMode="exactProteinMatch"><Segment range="3579-3632" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7534-7638" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1920" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5199-5320" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5339-5474" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2507" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4894-5127" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1933-2173" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase III promoter for human U6 snRNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2520-2788" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4233-4820" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5914-6502" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3633-4232" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4232" maxFusedRunOn="4232"><Segment range="2847-3509" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7464" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6673-7464" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7465-7533" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  e<Notes>
<UUID>475c61bc-cb47-4196-a062-7d76a159d9f7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="6:8:12">2023.6.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  7Z        š        šs"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕkUªlg’>jý†¾¶j6gÔ8¤De¤ZZ‡‰]Õ¤�Vr‘RYjè	*4ªU¦*•`y¶c§Éz&¬jè:™›¢Aí³“±jVÁ¼ÃÌPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.óë?´q,¿¨<ûüø'êQõ‡§PÓ‰¤÷ª•EP xZÞýqVQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Gª4GÕ¬–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�S           ‹  ‚  ¦  ¨  ™  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,<?xml version="1.0"?><Features nextValidID="24"><Feature recentID="23" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2770-2779" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4656" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4273" maxFusedRunOn="4273" detectionMode="exactProteinMatch"><Segment range="4657-4668" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8065-8081" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5489-5505" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5465-5481" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5558-5579" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5513-5519" color="#ffffff" type="standard"/><Segment range="5520-5537" color="#ffffff" type="standard"/><Segment name="-35" range="5538-5543" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3582" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4185" maxFusedRunOn="4185" detectionMode="exactProteinMatch"><Segment range="3532-3585" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7487-7591" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1920" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5152-5273" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5292-5427" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2249-2460" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4847-5080" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1933-2173" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase III promoter for human U6 snRNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2473-2741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4186-4773" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5867-6455" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3586-4185" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4185" maxFusedRunOn="4185"><Segment range="2800-3462" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7417" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6626-7417" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7418-7486" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  e<Notes>
<UUID>475c61bc-cb47-4196-a062-7d76a159d9f7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="6:7:25">2023.6.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ¨ý7zXZ  æÖ´F !   t/å£àU£e] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷¿Û{ãáp„&ÞóI1�wOþ@zÒlâÀvé ­',ÍžÌœ9‚´Æ@uæi\ïîŽ "°b—{6¸[‡ ¸†&Ú�tlòù5öî´/z…ÑÙMVÆÆñ»y"«œá1PÞÀ=Û¥TMþ.Z;É˜!ØšÜpÑ¼_>VòÎáåö¨ø­c†E—2™3¤ØxÏæí#ßð‰½8Zþ¹×’¡úé/ˆãõ†ØÒÙŠí—&1.?"ïz+¹À^ð…dDäÞ¯ßˆ¡Ìí”3y›CµAûÕk>VF çõaí@X}naÄˆ¾ì›r¡Ñx\’nB†’^;>Žzmbt´Þñã!ßo àesÈÔtö¸:'—åØæ£äß)F¶aì]Ö.6,<Çä«ÈÚá?`¦2CÏ.a_4?Ï]©Kéz)� iGK&3U‡õGLÓ`‹'ŽmÝV¯ÂwIÁˆïÍl’ÓóÞùp±•Å|á�î¨Ò®ÛÆê˜™iZœ,|) .íÖŒ;.UñeÅ¢!&µßa­"\+5�ùËÍ?Ñ•u±’¼ÕR†cFËÆa(;�%§á¾Q?a[vE‹K8>‹&R±W{Ð©&"A�£†]ÇŽ¯¦ùvÚš‡=4·æ�‰W«´°leùS8¸!EÌÕîyMJ1`88°9UdJÀÊò ý!àëûÞ¼ê#t*ƒ•Â…óã ¯	•ÅSµ5ëìïÄ‡\>•åG²iÐxì`ß,ÊÈi…¥%í–¢á-ÆP*TNsævÛ±¬–ywf˜À‹’ç_N�‡)XÛÊfSïÙšCR,D¾‚L­@×Á…±©M½”éÏàX‘™ü’Ä;Àü~¿'œÂÚ'.dÎÏL0¢ºñÛq¶s­Dðè6³¤¿‘TFãâæq€hîSž	Âƒú’.¬/Ú<?8ÅHÓÉ}Ìya,?ù@OÓð„e•Ôqø¤•ÚŠDŸÅPžõÅ�G²MÛóƒ{&å�µ·S¸ŠŽ�gêÇwÂŠÚ¿´Ž�W$3ÜþJ÷cdsÜ³´× ˆxéB^â†·ÃËUå'«’zbø�ô7—ÁÄ~ý+5»Ê‡{?¤üFÜÌT\± ·�gRHÎˆRÅHúÁÉ�œÏ5B™©õ½}¼ÖTë©ûdù‘cSD+ÅW$?n\�ž"SW�…50]_sÎ¯ñáSg¡6ñŽÔbi.|¶ ¥ž� |PhùþR˜ó®GÖ*{?ûž“Jýj]S·Ö?óq­Ï¥éá?°¢éÌ•ÿ”Œ¹ÓZw«ýõ~‹ŸãJð P<#XX+ è±º˜>Ê+ÕÊ‚`YÉG& ÊüÉ±¾8À¿’HM¦Ú7âY}ªè•
“reoK›=P˜Å’MÜŽ(ØÐæ�œø‡Ä] _eë 9ñÙèÉ:”¥J(Ù Ì³a~Mæv¹Ô�DÉë£xè¼ž½-k{Ésñ·Œð.J{­%*>8âŠï+Ô¢Ñ´Æü¹�(‘-{ù;
¸GAä'èýdúlkÌeÐ »/É¥qÜ(®§LûŠ©Ä€ÈØ*`“Ó“ô,e3,1Û6§“ëãî©ÈZþ@½À:¾®Ô›_t[àà“‰ ÕDA2B¤›
0€“ýMäñ(±®ñÊJ|žÚÚºƒ]­˜O†+Mx[Œ–ö¿=åñý^)Pítø>/‘Ui2_()¡˜‰=RrÄ#Š¢–H9WòK³SRÜüPÒ,éUÌ)°!d£ê0Fü'FŒžý7/{òLAŸB›Ä9ÍL<èÀ„gç/:Jöâh<¢!eõ!»Û.ef‹ëŸúA”c�}±q 0„Ö�ïtˆN˜›5ÊÏÏ@ôÂ�J4ß±Œ&�Uêr‹B”3Öa$Ük	]îüR<;Ž*rYi1Ë`×»›§·ŒÀ
Çi�½g]ÏÏ-÷A´Ò9J2B	l²:cÆ	²¨è÷®j!¦Æè4Íëc(*¯½¾kö4XSÊ•5EcÝ¬DBýöªi˜as&Ø92+C¾"àM
!I1_`hÿÒtJËýú£I² ÈÇëžÎ$)Fo©oºÌï`®p]ðÙ@!©1¬ç±R _¼Õ¡Èc <!µóðCŸQ‰Ú Ì1‚A"e'Ñ y—EaÒ§COŸÇê,~ºÐchcTuZrQvFJ©�ô/uTñZFô-ÿ/¨BDï¶lSvÍ¡(¸ž„¬+6¤¤ìžˆ§à%ù9�‰ö2
‰N‰lö½:µ Ÿ¢²{ÞÏŸP[‡%.×ñÞ²zYíXW¹Ž«’k+×ÿì|�eEt2˜ér^½��æ0t¾‡Í†T#ÍŸF|­|¼RîmÙÍÑÿÆ±»A¼ÛZãÜ9ìÖA Ú¸HH/ïàÑ™'G'[îcjðÚKÁ(Z�õçï½b¢¢²ˆk¡
Cá�BŸV�¹Ú-¥ê¿ù‡Úí×úà©$ò¼Å4{¶ÿÕpóZ€ñ—}X:h\Ð•±¼–‡å´kuí¢®žæV4ª,CÿY ÐJø­ZÙÿë.2¡tÔõN-4Wò­‰!óÍ~>ˆfÌÒ¦Y·Üñ�(†yä¡mÁ^n©_g8™nÍ*ßÒ"0Ô+°€±bÆÎö6%��}úzÃRQ“VœpÆÑt˜1+ûžd©X‰vcýŸ`²>´é D®ù“¾üüÏH~dk×¢»úAôS©ü#¸‰û,5fˆ†•iËºèFlË«ä‘’†²öítëÓèøðú.\™I~oˆÍé%ž¤ô¶Ôä C›üeY§i„y7â]wþÕÀšWW8“÷Ðf©$êÞùXZ§ÉW“§Ÿ¢f¤À/¯/êw R½»sd– KN—º˜ñ¦pG(n†-ŠÔ5H5ô?ƒ-µ¶=xÚöàÕc�¢mü�gùC(M`ðÀjúj1 <%Ý	íS}ÛþÍz%|#;húåÓQÀ‚Ä,Ì¨¾"Y	•Î™<ÖH±x™þžÂï0Ø«Þª<è‹@ŒÝÄé*¼9M–¥Øq"þùå¬æjfàÓ;™ëÜïë¨þ¼ò4 !]W©ébPÆj$cCò¯}?åwyáãT´#A<üªÂ¢óp¥Ì°ò¬–÷»U1€”X)�ƒgÑ{PÜCñnžó¨±iQÒ™õÈk ±:2ƒÔµü:1`ÌëEw{šì ©€kØC�’�ÿ˜¾�‹è�%HT�—ýZ$ì‚?¬ËõM=¿TÞ
ž5�÷¤Î¶ Š÷¥ö<–ÓWJ_ÒëSÅ‚dÛÍ¼‹¯J³ånqšjFL‘Õ8£¤ëó¹.Cãkb‰¸æ|À5+R,wç:h‚ºÃXD0@r1-ù©+ò29ð£|‡øÙ� D¬¯ÓlÒ9,·##/‡~|Ö—¨Ï£lŸÀóIïõz'-ÔíÂ’
ÐÓ%_?\Çx³S¾5Yà•°^—ãÅƒ
äÿ^¹Æ° KåÉØeO¨Ü©³…½ÐÜò“wÏÆŸ©ƒFç.´ÖÌ
¥	I{aEóCé„¼$ñ|]Vx­C•?ÄÁŽŸì}±ÇaGdÞTÉ1Àü`âÝåT¶z„C©Ã¤•Q¢ž-i‚äÿ:¿[»sp<N"g.‘S_ÕÖ<Ì¯Ü¾¯=>2ƒlŽeãï)l—hQõfS5�˜²��ó”„ýKÃxtW*$)C_Yßó* ŸŸ{˜ÎE.G|{[äÝ™û      »âÎ'£øw\ �¤«  UFÊ±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0\<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7464" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6673-7464" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7465-7533" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="1" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4232" maxFusedRunOn="4232"><Segment range="2847-3509" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3633-4232" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5914-6502" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4233-4820" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2520-2788" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1933-2173" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase III promoter for human U6 snRNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4894-5127" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2507" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5339-5474" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5199-5320" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1920" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7534-7638" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3629" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4232" maxFusedRunOn="4232" detectionMode="exactProteinMatch"><Segment range="3579-3632" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EGRGSLLTCGDVEENPGP"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5560-5566" color="#ffffff" type="standard"/><Segment range="5567-5584" color="#ffffff" type="standard"/><Segment name="-35" range="5585-5590" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5605-5626" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5512-5528" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5536-5552" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8112-8128" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4703" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4320" maxFusedRunOn="4320" detectionMode="exactProteinMatch"><Segment range="4704-4715" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="23" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2817-2826" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="24" name="shRNA2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2192-2242" color="#a6acb3" type="standard"/></Feature></Features>
  Š<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="R1" sequence="gatccAAAAAAGCCTACCTGAGGATGAAGTATTCTCTTGAAATACTTCATCCTCAGGTAGGCcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2189-2252" boundStrand="1" annealedBases="gatccAAAAAAGCCTACCTGAGGATGAAGTATTCTCTTGAAATACTTCATCCTCAGGTAGGCcg" meltingTemperature="70"><Component hybridizedRange="2189-2252" bases="gatccAAAAAAGCCTACCTGAGGATGAAGTATTCTCTTGAAATACTTCATCCTCAGGTAGGCcg"/></BindingSite><BindingSite simplified="1" location="2189-2252" boundStrand="1" annealedBases="gatccAAAAAAGCCTACCTGAGGATGAAGTATTCTCTTGAAATACTTCATCCTCAGGTAGGCcg" meltingTemperature="70"><Component hybridizedRange="2189-2252" bases="gatccAAAAAAGCCTACCTGAGGATGAAGTATTCTCTTGAAATACTTCATCCTCAGGTAGGCcg"/></BindingSite></Primer><Primer recentID="1" name="F1" sequence="aattcgGCCTACCTGAGGATGAAGTATTTCAAGAGAATACTTCATCCTCAGGTAGGCTTTTTTg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2185-2248" boundStrand="0" annealedBases="aattcgGCCTACCTGAGGATGAAGTATTTCAAGAGAATACTTCATCCTCAGGTAGGCTTTTTTg" meltingTemperature="70"><Component hybridizedRange="2185-2248" bases="aattcgGCCTACCTGAGGATGAAGTATTTCAAGAGAATACTTCATCCTCAGGTAGGCTTTTTTg"/></BindingSite><BindingSite location="5457-5467" boundStrand="0" annealedBases="AGGCTTTTTTg" meltingTemperature="34"><Component bases="aattcgGCCTACCTGAGGATGAAGTATTTCAAGAGAATACTTCATCCTCAGGT"/><Component hybridizedRange="5457-5467" bases="AGGCTTTTTTg"/></BindingSite><BindingSite simplified="1" location="2185-2248" boundStrand="0" annealedBases="aattcgGCCTACCTGAGGATGAAGTATTTCAAGAGAATACTTCATCCTCAGGTAGGCTTTTTTg" meltingTemperature="70"><Component hybridizedRange="2185-2248" bases="aattcgGCCTACCTGAGGATGAAGTATTTCAAGAGAATACTTCATCCTCAGGTAGGCTTTTTTg"/></BindingSite><BindingSite simplified="1" location="5457-5467" boundStrand="0" annealedBases="AGGCTTTTTTg" meltingTemperature="34"><Component bases="aattcgGCCTACCTGAGGATGAAGTATTTCAAGAGAATACTTCATCCTCAGGT"/><Component hybridizedRange="5457-5467" bases="AGGCTTTTTTg"/></BindingSite></Primer></Primers>
  ×<Notes>
<UUID>f2ae5f49-634d-414d-9a2a-2eb7f8aa5816</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pLV3-U6-DPP7(human)-shRNA2-CopGFP-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="19:36:10">2025.7.8</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      HO ÿþ               E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
